货号：  A14528

Gibco™  相关应用： 哺乳动物细胞培养 | 蛋白质和抗体生产 | 蛋白质表达系统和载体

Expi293F™ human cells are derived from the 293 cell line, and are a core component of the Expi293™ Expression System. They are maintained in suspension culture and will grow to high density in Expi293™ Expression Medium. Expi293F™ cells are highly transfectable and generate superior protein yields compared to standard 293 cell lines in transient protein expression.

• Suspension culture cells pre-adapted to Expi293™ Expression Medium

• Demonstrated higher protein expression levels than other 293 cells adapted to Expi293™ Expression Medium

• Exhibit more rapid cell growth and higher culture viabilities than standard suspension-adapted 293 cells

• Consistent transient expression performance of Expi293F™ cells for over 40 passages beyond thaw

Frozen cells are supplied in a vial containing 1 ml of cells at 1 x 107 viable cells/ml in Expi293™ Expression Medium and 10% DMSO. The cells should be thawed directly into Expi293™ Expression Medium. The six-vial multipack eliminates the need to expand and rebank the cells in the lab.

Cells are typically grown in flasks on a shaker platform in a humidified, CO2 cell culture incubator. However, cultures are scalable from volumes of