货号：  0010057AE

Gibco™  相关应用： 哺乳动物细胞培养 | 蛋白质和抗体生产

描述

GIBCO® Anti-Clumping Agent is a concentrated liquid reagent that reduces cell clumping to assist in attaining higher viable cell densities in suspension culture. Anti-Clumping Agent is a gentle, animal origin-free, chemically defined formulation that contains no proteins, enzymes, hydrolysates, or components of unknown composition.

• Reduced cell clumping in suspension culture

• Higher viable cell densities

• Animal origin free (AOF) and chemically defined components

• Convenient packaging with a larger size now available

• Customization available to configure packaging and delivery

Simple and Gentle Cell Dissociation

Cell lines adapted to suspension culture can revert to a more adherent or aggregated “clumping" state. When this occurs, culture condition can be greatly improved by adding a gentle reagent to reduce clumping and adhesion instead of the harsh enzymes often found in cell dissociation products. Anti-Clumping Agent is a simple, gentle, concentrated solution which, when added to culture media (usually titrated between a 1:100 and 1:1000 dilution), can reduce cell clumping significantly. 

Reduced Clumping, Increased Viability, Improved Protein Expression

Reduced cell clumping achieved through use of Anti-Clumping Agent is illustrated below (Fig 1). See also the data demonstrating improved viable cell density (Fig 2). Higher viable cell density contributes to the stabilization of culture conditions for better protein yield. 

For actual cell dissociation, we recommend our animal origin-free TrypLE™ products. 

Use Anti-Clumping Agent after Transfection

Anti-Clumping Agent is not recommended in all suspension cell culture conditions. For example, when creating a cell line using lipid cationic transfection, Anti-Clumping Agent can interfere with the transfection process, so in this case we recommend that Anti-Clumping Agent be used after transfection. 

Animal Origin Free and Chemically Defined Components

Because Anti-Clumping Agent is made with only animal origin free and chemically defined components, you can be assured that the risk of adding adventitious agents to your culture system is minimized. Avoiding additives in your system that are not well defined can lead to more robust results from batch to batch.