Features of this affinity matrix include:
• IgM purification from plasma and serum
• Purification of monoclonal IgM from cell cultures
• Efficient purification of IgM with high yields and retained activity
• Excellent scalability
• Non-animal derived
• High flow rates
• Cross-reactivity towards mouse and rat IgM
POROS CaptureSelect IgM Affinity Matrix binds to the Fc part of IgM. The high selectivity and yields obtained using IgM Affinity Matrix enable a robust, fast, and efficient purification process with excellent purity obtained in one step.
Free of animal components
Thermo Fisher Scientific POROS CaptureSelect products contain affinity ligands created by a proprietary technology based on camelid-derived single-domain antibody fragments. The ligand is a 13-kDa single-domain fragment that comprises the 3 CDRs that form the antigen binding domain and is efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.
Main characteristics:
Matrix: Cross-linked poly(styrene-divinylbenzene)
Average particle size: 50 µm
Ligand: CaptureSelect IgM affinity ligand
Ligand coupling method: Carbonyldiimidazole (CDI)
Binding capacity: ~6 g/L resin (determined with polyclonal human IgM from serum)
Elution conditions: Acidic elution with 0.1 M glycine, pH 3.0
Mechanical resistance: 100 bar (1,450 psi, 10 MPa)
Formulation buffer: 20% (v/v) ethanol
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